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Product code: Biology-BH09


ABIO425 – Molecular Biology

You performed an immune blot/western blot for your protein of interest from three different cell lines and observe the results below. One cell line, cell line #1, shows your protein of interest running at approximately 17 kD in size. However, when extracted from cell lines #2 and cell lines #3 the protein of interest runs close to 43 kD.


1. Within the mechanisms of gene expression covered during class, what mechanism could explain the observed the result. Please describe your hypothesis and briefly explain why your proposed mechanism can explain the observed result.

2. What experiment could you perform to test your hypothesis? Please describe your experiment, and the outcome you expect.

3. Based on the results above, what mechanism of gene expression covered during class is most likely not at work here. Please briefly explain why.


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